About Tr1X
Tr1X is a clinical-stage biotechnology company developing engineered Tr1 cell therapies to restore immune balance in autoimmune and inflammatory diseases. Leveraging the unique biology of Tr1 cells, the company is advancing a pipeline of off-the-shelf allogeneic and in vivo CAR-Tr1 therapies designed to deliver targeted immunoregulation and address diseases driven by immune dysfunction, including transplant-related complications. Founded by pioneers in the discovery of Tr1 cells, Tr1X, Inc. is focused on developing durable, potentially curative therapies for conditions with high unmet need. For more information, visit www.tr1x.bio.
Position Summary
The Research Associate II (RA II) performs a variety of lab responsibilities in support of translational assay development and clinical sample analysis for ongoing and upcoming clinical trials. The RA II possesses a general technical understanding and foundation, strong work discipline, exceptional attention to detail, and the ability to apply critical thinking to generate reproducible, high-quality data that inform the translational development of Tr1X’s cell therapy programs. The RA II is expected to operate with a level of general knowledge and independence but will perform duties with oversight and guidance from Translational scientists. This role involves hands-on work with human blood and tissue specimens.
Key Responsibilities
Assay Development and Clinical Sample Processing
Process human blood and tissue samples according to SOPs and established workflows.
Perform weekly flow cytometry runs, including sample preparation, instrument setup (QC, compensation, PMT adjustments), data acquisition, and troubleshooting.
Complete and deliver real-time data analysis, ensuring accuracy, reproducibility, and adherence to assay requirements.
Conduct MSD/ELISA and molecular assays (e.g., dPCR) as needed.
Maintain cell cultures and perform cell-based assays.
Support assay development, optimization, qualification, and troubleshooting in collaboration with scientists.
Prepare concise summaries of experimental design and results; deliver presentations in team settings.
Ensure accurate, real-time documentation of all activities using an electronic lab notebook (ELN).
Learn, develop and enhance lab skills, troubleshooting capabilities, and knowledge through scientific curiosity and support from more senior level scientists.
Supportive Translational Responsibilities
Contribute to SOP drafting, updates, method documentation, and controlled form revisions.
Participate in clinical sample management including receipt, logging, storage, aliquoting, and preparation for downstream analyses.
Maintain detailed documentation, chain-of-custody, and compliance expectations for clinical samples.
Perform routine lab upkeep such as reagent inventory.
Perform other responsibilities based on business needs.
Required Qualifications & Experience
Bachelor’s degree in immunology, cell biology or a related scientific field.
Minimum 2+ years of industry or equivalent experience in assay development, immunology, cell biology, or a related field, ideally within a translational or biomarker-focused setting.
Hands-on experience with flow cytometry–based assays, including sample processing, instrument setup, data acquisition, data analysis, and troubleshooting; experience with panel design is a plus.
Hands-on experience in maintaining cell cultures on primary human cells.
Experience executing cell-based assays, such as mixed lymphocyte reactions (MLRs), is a plus.
Demonstrated ability to reproducibly conduct multiplex immunoassays (e.g., ELISA, MSD) and/or molecular assays.
Proficiency with analytical software programs (Prism, FlowJo, etc.).
Strong attention to detail with the ability to follow SOPs and maintain real-time, accurate documentation in ELNs.
Familiarity with assay development, qualification, and validation workflows, and knowledge of GxP principles or compliance expectations relevant to clinical…