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Posted Job Title
Research Specialist A/B
Job Profile Title
Research Specialist A
Job Description Summary
The Amado Laboratory, a part of both the Perelman School of Medicine at the University of Pennsylvania and the Children’s Hospital of Philadelphia, is seeking a highly motivated Research Specialist A/B to join our research program focused on the development of gene therapy approaches to treat ALS and related neurodegenerative disorders.
Job Description
Our lab uses cellular and mouse models of disease to identify therapeutic targets and develop cutting-edge gene therapy-based approaches to treat them, including AAV-mediated gene delivery, RNA interference, gene editing, and ASOs. We are seeking someone who can help design, conduct, and analyze experiments in immortalized cell lines, patient-derived stem cells, and mouse models of diseases of the nervous system in a highly collaborative, fun environment. The ideal candidate is comfortable with basic existing lab techniques and is also keen on reading published literature to identify the most effective, reliable, and novel strategies to advance the projects’ success, and is not afraid to try new things and troubleshoot. Excellent organization and record-keeping skills are required. Prior experience with the following techniques is desired: DNA/RNA/protein extraction, QPCR, western blotting, molecular cloning, PCR, and cell culture including maintenance and transfection. Prior experience with mouse handling and knowledge of or willingness to learn tissue extraction techniques (especially brain, spinal cord, and/or peripheral nerve) and behavior testing is preferred, as there is a significant in vivo component to our work.
Sectioning/immunohistochemistry/immunofluorescence experience is a bonus, as is experience with imaging acquisition and analysis. For Research Specialist B applicants, duties will be more autonomous with a greater level of independence. Further, although both positions are expected to lead to inclusion in publications from the lab, a Research Specialist B will also be expected to assist in generating figures, writing Methods, and other aspects of manuscript preparation.
Qualifications
RS-A: Bachelor of Science and 0 to 1 year of experience or equivalent combination of education and experience is required.
Prior experience with mammalian cell culture (including transfection) and molecular/cell biology is preferred.
Prior experience working with mice is preferred; openness to it is required.
Ability to work both independently and as part of a team are required.
Excellent organizational, time…